Identification of Intestinal Microbial Community in Gallstone Patients with Metagenomic Next-Generation Sequencing

Gallstone disease (GD) is one of the most common gastrointestinal diseases worldwide. Nowadays, intestinal microbiota are thought to play important roles in formation gallstones. In our study, human fecal samples were extracted for metagenomic next-generation sequencing (mNGS) on Illumina HiSeq platform, followed by bioinformatics analyses. Our results showed that there was a particular micro-ecosystem GD patients. contrast healthy people, sequences Bacteroidetes, Bacteroides and Thetaiotaomicron obviously more abundant patients at phylum, genus species levels, respectively. On other hand, glycan metabolism drug resistance, especially β-lactams, profound functions gut microbes compared those normal subjects. Furthermore, correlation analysis drew out existed significant relationship between serum levels biochemical indicators abundances illuminate both composition All all, study can broaden insight into potential mechanism how affect progression gallstones some extent, which may provide targets prevention, diagnosis or treatment GD.